Bial's test

Bial's test is a chemical test for the presence of pentoses. The components include orcinol, hydrochloric acid, and ferric chloride. In the presence of a pentose, the pentose will be dehydrated to form furfural. The solution will turn bluish and a precipitate may form. The solution shows two absorption bands, one in the red between B and C and the other near the D line [1].

Composition

Bial's reagent consists of 0.4gm orcin, 200 ml of concentrated hydrochloric acid and 0.5 ml of a 10% solution of ferric chloride [2].

References

  1. ^ Baldwin, E. and Bell, D.J., Cole's Practical Physiological Chemistry, published by Heffer, Cambridge, 1955, page 189
  2. ^ Baldwin and Bell, page 189